Education | Method development | Onsite training
Affinity | ion exchange | gel filtration | IMAC | RP HPLC | HILIC | chromatofocusing |
New chromatography methods:
CCAEC | conformation | minor component removal | misfolded protein removal | monoclonal therapeutic antibody deamination removal | PEG isoform removal | recombinant protein isoform and protein 3D structure based purification | separation of lipid-bound from lipid-free BSA | separation of holo and apo lactoferrin | separation of whey proteins | CPNPC |
CCAEC & CPNPC
align to the quantum field
optimization of processing or analytical conditions
The new methods provide a game changer in protein isolation and analysis. Chromatofocusing resolution in an ion exchange setup. A cost-effective approach and reduces the number of steps, decreases costs, improves purity and yields. Removes minor contaminating components, misfolded proteins, and derivatives that have differences in shape or iso-electric point, differences in minerals bound and the presence of co-factors or other bound ligands that alters either the shape or iso-electric point of the protein.
If you want to have another set of eyes cast over your process and analysis method to understand why things are not working as you desire. I can then compare this to the new techniques involving CCAEC and CPNPC. I could take a look at your target with the intent to see if I could develop a more cost-effective approach using CCAEC and conformational chromatography or CPNPC methods.
analytical & industrial
CCAEC has the resolving power of chromatofocusing (separating proteins with 0.1 pH unit difference for their isoelectric points) but the price point of ion exchange. CCAEC is a quantum step forward in protein purification. Proof of concept demonstrated by separating beta-lactoglobulin A and B from whey.
Economic benefits of using CCAEC
Reduce the number of steps to purify the target protein of interest and reduce processing costs. Creates a better value proposition.
Improve yields and purity. Improves economic performance. Reduces waste, therefore, more economically sustainable.
Target the removal of minor components of antibody deamination products. Monoclonal therapeutic antibodies. Use conformational chromatography methods to separate different folding structures which changes the game when it comes to recombinant misfolded protein separation. No difference in isoelectric points but different three-dimensional structures can be separated.
Proof of concept demonstrated using apo and holo lactoferrin separation using CCAEC and conformational chromatography.
I have developed a method that allows the analysis of iron content in the different forms of lactoferrin by separating lactoferrin into iron free, N lobe iron bound, C lobe iron bound and N and C lobe iron bound using a combination of CCAEC and conformational chromatography.
lipid bound and lipid free BSA fractionation
CCAEC has been successfully applied to the fractionation of BSA into different forms depending on the presence of bound lipid or free lipid and the presence of bound bilirubin.
holo & apo lactoferrin separation
The use of CCAEC and conformation chromatography has enabled separation of holo and apo forms of lactoferrin which has not been possible previously.
If you are interested in understanding the bioactivity of the energy generating system in whey then you need to understand the iron content of lactoferrin and the lactoperoxidase system.
ICP MS, Randox and the Ratio methods are not able to tell you what percentage of lactoferrin contains one atom of iron in the N or C lobe and what percentage of lactoferrin is iron free or contains two high-affinity irons bound. If this is something that you are interested in then please get in contact with me.